Impact of provitamin A biofortified maize consumption on carotenoid status of Zambian children

Abstract Number Theme Presentation Type Cover Approved
0505 Efficacy or effectiveness of micronutrient interventions Poster Not Approved


Abstract Content


Provitamin A carotenoid biofortified “orange” maize has been developed as a vitamin A deficiency control strategy using selective breeding for the ß-carotene hydroxylase 1 gene, which increases ß-carotene, ß-cryptoxanthin, and zeaxanthin in the endosperm. We conducted a cluster-randomized trial to test the impact of orange maize consumption (a predominantly ß-carotene-rich variety) on serum retinol concentration in rural Zambian children (4-8 y). In the present analysis, we tested the intervention’s impact on circulating carotenoids.


Children (n=1024, 4-8 y) were cluster-randomized to receive 200 g dry weight/d orange or conventional “white” maize prepared with standardized low-vitamin A relishes, 6 d/w for 6 m. The carotenoid profile was assessed in baseline and follow-up serum samples in a subset (n=321 white, n=358 orange) of children. We assessed differences in log-transformed carotenoids, expressed as geometric means and 95% confidence limits, accounting for cluster randomization.


Baseline carotenoids did not differ between groups. There were no differences in lycopene and lutein between groups at follow-up. Other carotenoids increased significantly (p<0.001) in orange vs white groups following the six-month intervention: ß-carotene [273 (254,292) vs 147 (135,160) nmol/L], a-carotene [19.7 (18.4,21.1) vs 10.6 (9.9,11.3) nmol/L], ß-cryptoxanthin [36.1 (33.6,38.8) vs 12.6 (11.8,13.5) nmol/L], a-cryptoxanthin [18.7 (17.4,20.1) vs 8.9 (8.4,9.4) nmol/L], zeaxanthin [127 (119,135) vs 81 (75,88) nmol/L].


Children regularly consuming a biofortified maize variety bred primarily for ß-carotene had higher concentrations of other provitamin A carotenoids and zeaxanthin, suggesting the potential for health benefits beyond vitamin A deficiency control. Supported by HarvestPlus.

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