Validation of a semi-quantitative test for Vitamin A detection in coconut cooking oil

Abstract Number Theme Presentation Type Cover Approved
0342 Innovations in methods for monitoring and surveillance of programs Poster Not Approved


Abstract Content


To assess the validity of QuickView to estimate retinyl palmitate and to classify coconut oil as adequately fortified.


The QuickView Vitamin A Test Kit estimates vitamin A content in oil from 5-25 mg RE/kg (in increments of five) based on the Carr-Price reaction. High Performance Liquid Chromatography (HPLC) was the reference method. Linearity and agreement was described. Combinations of QuickView concentrations were used to predict the fortification status and minimum thresholds. A sample size of 100 allowed for the estimation of a specificity of 85%. Fortification status was based on Philippine implementing rules and regulations (IRR) : 1) IRR (12 – 23 mg RE/kg) and 2) IRR20 (9.6 – 27.6).


Linearity was defined by y=0.9317 + 5.04x ; R2 is 0.66. The mean difference was -4.036 mg RE/kg. The observed agreement was 64% (IRR) and 92% (IRR20) with kappa(IRR) = 0.22 (p<0.01) and kappa(IRR20) = 0.67 (p < 0.0001).The sensitivity and specificity were 86% and 85% with QV 7 (10 - 20 mg RE/kg) and 95% and 71% with QV 6 (10-15 mg RE/kg) against IRR20. Against IRR, specificity was above 90% if sensitivity was 60%. QuickView performs best (80-100% sensitivity and specificity) when used to identify a minimum threshold of 10 or 15 mg RE/kg without taking into account a ceiling level.


The kit is best used to estimate minimum thresholds of vitamin A content in fortified coconut cooking oil.

Our website uses cookies to ensure you have the best experience.
Please visit our Privacy Policy page for more information.