Validate a non-invasive method (Masimo Pronto 7) for the determination of hemoglobin concentration in children from one to five years of age in San Luis Potosí, México.
A study was developed in order to validate hemoglobin measurements from the Masimo Pronto 7. A sample of 148 children with 3 repeated measurements were performed with the Masimo Pronto 7 device, which measure hemoglobin through absorption of visible or infrared light. Also a sample of 5 ml of venous blood in a vacuumed tube with potassium EDTA as the anticoagulant was obtained. Three repeated measurements of hemoglobin with HemoCue Hb 201 was obtained device as a comparing equipment. The Mindrey BS 200 automated equipment with Pointe Scientific reactive was used as the gold standard hemoglobin measurement. The concordance correlation coefficient was used to evaluate measurement reproducibility.
Masimo Pronto 7 showed an average of 12.6 g/dL of hemoglobin (95% 12.5, 12.8), HemoCue average was 12.9 g/dl (95% 12.8, 13.1) and gold standard was 15.0 g/dL (95% 14.8, 15.3). The concordance correlation coefficient for Masimo was 0.104 (p < 0.001), with a precision of r = 0.279 and an accuracy of b = 0.371. The HemoCue, has a concordance coefficient of 0.207 (p < 0.001), with a precision of r = 0.552 and accuracy of C_b = 0.375.
Masimo Pronto 7 and the HemoCue, are biased downward, this represents an underestimation of hemoglobin concentrations compared to the gold standard; both devices have almost the same bias correction factor.